PARP1 antibody (acLys521)

Catalog No. ABIN7862473

The Rabbit Polyclonal anti-PARP1 antibody (ABIN7862473) specifically detects PARP1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PARP1 antibody (acLys521) (ABIN7862473)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PARP1 Antibody

Binding Specificity: acLys521

Purpose: Rabbit Anti-PARP-1 (Acetyl-K521) Polyclonal Antibody

Specificity: The antibody detects endogenous levels of PARP-1 when Acetyl occurs at Lys521(human/rat) or Lys520(mouse), while it doesn't react with total protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human PARP-1 (Acetyl-K521) protein at AA range: K521

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP-1

Background: Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+ ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1PARP1 encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. PARP1 (Poly(ADP-Ribose) Polymerase 1) is a Protein Coding gene. Diseases associated with PARP1 include Diphtheria and Hemorrhagic Cystitis. Among its related pathways are Necroptosis and Telomere C-strand (Lagging Strand) Synthesis.

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location