ACTH antibody (AA 1-24)

Catalog No. ABIN7865553

The Mouse Monoclonal anti-ACTH antibody is suitable to detect ACTH in samples from Rat. It has been validated for IHC, ELISA, IF, FACS and ICC.

Quick Overview for ACTH antibody (AA 1-24) (ABIN7865553)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACTH antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 2F6

Product Details anti-ACTH Antibody

Binding Specificity: AA 1-24

Purpose: anti-ACTH mouse monoclonal, 2F6, purified

Purification: affinity chromatography

Immunogen: ACTH synthetic peptide (aa 1-24) conjugated to KLH

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Alternative Name: ACTH

Background: ACTH (same as corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. 2F6 is specific to synacthen (aa 1-24 of ACTH), does not react with CLIP (aa 17-39 of ACTH). ACTH is also produced by cells of the immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Positive control: Pituitary gland, pituitary tumor.

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity