Blood Group Lewis B antibody

Catalog No. ABIN7865568

The Mouse Monoclonal anti-Blood Group Lewis B antibody is suitable to detect Blood Group Lewis B in samples from Human and Guinea Pig. It has been validated for IF, WB, ELISA, IHC, FACS and ICC.

Quick Overview for Blood Group Lewis B antibody (ABIN7865568)

Target: Blood Group Lewis B

Reactivity: Human, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Blood Group Lewis B antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 2-25LE

Product Details anti-Blood Group Lewis B Antibody

Purpose: anti-Lewis b mouse monoclonal, 2-25LE, purified

Purification: affinity chromatography

Immunogen: ALea-b+ ovarian cyst mucin

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Blood Group Lewis B

Target: Blood Group Lewis B

Alternative Name: Lewis b

Background: 2-25LE reacts with Lewis b antigen which is highly expressed in stomach, colon, small intestine, lung and kidney and to a lesser extent in salivary gland, bladder, uterus and liver. Positive Control: Stomach, colon, small intestine, lung and kidney, colorectal adenocarcinoma.