CD45RB antibody

Catalog No. ABIN7865594

This Mouse Monoclonal antibody specifically detects CD45RB in FACS, IHC, WB, IF, ELISA and ICC. It exhibits reactivity toward Human.

Quick Overview for CD45RB antibody (ABIN7865594)

Target: CD45RB

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RB antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Clone: Bra11 (Bra11G)

Product Details anti-CD45RB Antibody

Purpose: anti-CD45RB mouse monoclonal, Bra11 (Bra11G), purified

Cross-Reactivity (Details): negative with rat, rhesus monkey

Purification: affinity chromatography

Immunogen: non-T, non-B CALLA positive ALL cell line REH

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL)

WB_dilution: assay dependent

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD45RB

Target: CD45RB

Alternative Name: CD45RB

Background: CD45 glycoproteins have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out). Bra11 reacts with CD45RB, which is expressed on mature B-lymphocytes and the majority of lymphomas and leukemia's of B-cell origin. CD45RB is also expressed on cells of myeloid lineage. Positive control: Ramos, U-698, or GA-10 cells. Tonsil.