CD59 antibody

Catalog No. ABIN7865599

The Mouse Monoclonal anti-CD59 antibody (Clone Bra10G) (ABIN7865599) specifically detects CD59 in IHC, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for CD59 antibody (ABIN7865599)

Target: CD59

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD59 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: Bra10G

Product Details anti-CD59 Antibody

Purpose: anti-CD59 mouse monoclonal, Bra10G, purified

Purification: affinity chromatography

Immunogen: K-562 leukemia cells

Isotype: IgG2b kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD59

Target: CD59

Alternative Name: CD59

Background: CD59, or protectin, is a 18-22 kDa cell surface molecule on a GPI anchor. It regulates complement-mediated cell lysis and is supposed to protect normal and tumor cells from cytotoxic attack by homologous complement through binding to C8 and C9. CD59 is expressed on leukocytes, vascular epithelium, a variety of epithelial cells and placenta. B-cells express low levels. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease Paroxysmal nocturnal hemoglobinuria (PNH). Bra10G was typed at the VIth International Workshop on human leucocyte differentiation antigens. Positive control: Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, human lymphocytes, human lymph node and tonsil.

Pathways: Complement System