CD8B antibody

Catalog No. ABIN7865609

This Mouse Monoclonal antibody specifically detects CD8B in FACS, IHC, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for CD8B antibody (ABIN7865609)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: EBS-CD-007

Product Details anti-CD8B Antibody

Purpose: anti-CD8b mouse monoclonal, EBS-CD-007, purified

Purification: affinity chromatography

Immunogen: human PBL

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8b

Background: EBS-CD-007 recognized a protein of 32 kDa, identified as CD8b. The CD8 Molecule consists of α and β chains, which are disulphide-linked into heterodimers or homodimers. CD8 is expressed on a T-cell subset (cytotoxic-suppressor T-cells), thymocytes and NK cells. The majority of CD8+ T-cells express CD8 as heterodimer. Some subpopulation of CD8+ T-cells as well as NK-cells may express homodimer. CD8 functions as a co-receptor is concert with TCR for binding the MHC class I/peptide complex. HIV-2 envelope glycoprotein binds CD8 α chain but not β chain. Positive control: HuT78 or hPBL. tonsil.

Pathways: TCR Signaling