CD99 antibody

Catalog No. ABIN7865611

The Mouse Monoclonal anti-CD99 antibody (Clone EBS-CD-044) (ABIN7865611) specifically detects CD99 in IHC, WB, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for CD99 antibody (ABIN7865611)

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: EBS-CD-044

Product Details anti-CD99 Antibody

Purpose: anti-CD99 mouse monoclonal, EBS-CD-044, purified

Purification: affinity chromatography

Immunogen: purified E-rosette forming cells from human PBL

Isotype: IgM kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,02 % saponin or digitonin for 15 min at 4 °C

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD99

Target: CD99

Alternative Name: CD99

Background: EBS-CD-044 reacts with CD99 or MIC2. Human thymocytes, PBLs and some T-ALL isolates and cell lines are positive. It is also present on pancreas and on Ewing sarcoma, which forms the practical application of the antibody. It is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Positive control: Pancreas, thymus, Ewing sarcoma.