CD21 antibody

Catalog No. ABIN7865617

The Mouse Monoclonal anti-CD21 antibody (Clone B-G11) (ABIN7865617) specifically detects CD21 in FACS, IHC, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for CD21 antibody (ABIN7865617)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD21 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: B-G11

Product Details anti-CD21 Antibody

Purpose: anti-CD21 mouse monoclonal, B-G11, purified

Purification: affinity chromatography

Immunogen: human PBL

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21

Background: B-G11 reacts with D21, a 140 kDa cell surface molecule which acts as a receptor for EBV, for human complement factor C3d (CR2) and for IFN-alpha. It is a glycoprotein, made up of multiple (n=15) Short Consensus Repeats (S.C.R.) sequences. CD21 is expressed strongly on mature B-cells, follicular dendritic cells and weakly on immature thymocytes and T-lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression also gradually lost after stimulation of B-cells in vitro. Positive control: Human PBL and tonsil.

Pathways: Complement System