Fibronectin antibody

Catalog No. ABIN7865633

This Mouse Monoclonal antibody specifically detects Fibronectin in WB, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Pig, Dog and Ferret.

Quick Overview for Fibronectin antibody (ABIN7865633)

Target: Fibronectin

Reactivity: Human, Pig, Dog, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 616

Product Details anti-Fibronectin Antibody

Purpose: anti-Fibronectin mouse monoclonal, 616, purified

Purification: affinity chromatography

Immunogen: high molecular fraction of proteins secreted by cultured human fibroblasts

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL)

WB_dilution: 1:100 (1 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Fibronectin

Target: Fibronectin

Background: 616 Reacts with the N-terminal fibrin and heparin-binding domain. Specificity was established by Western blotting with purified 29 kDa domain from two different sources. Fibronectins, 220 kDa monomer, 440 kDa dimer, are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. Positive control: Human kidney.