Integrin beta 3 antibody

Catalog No. ABIN7865663

This Mouse Monoclonal antibody specifically detects Integrin beta 3 in IHC, FACS, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for Integrin beta 3 antibody (ABIN7865663)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: EBS-CD-034

Product Details anti-Integrin beta 3 Antibody

Purpose: anti-CD61 mouse monoclonal, EBS-CD-034, purified

Purification: affinity chromatography

Immunogen: stimulated human PBMC's

Isotype: IgM kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: CD61

Background: EBS-CD-034 reacts with a complex of CD41 and CD61, i.e. alpha IIb integrin and the integrin beta chain. The MW of the GPIIIa is 105 kDa unreduced and 90 kDa reduced. The integrin beta 3 chain can also form a complex called the vitronectin receptor with integrin alpha V: the CD51/CD61 complex. Ligands are fibrinogen, fibronectin, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The CD51/CD61 complex is also found on endothelial cells, some B-cells, monocytes/macrophages and tumor cells. Positive control: U937, KG1a, HEL cells, human platelets in lymph nodes or tonsils.

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex