ICAM-3/CD50 antibody

Catalog No. ABIN7865666

This Mouse Monoclonal antibody specifically detects ICAM-3/CD50 in FACS, IHC, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for ICAM-3/CD50 antibody (ABIN7865666)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM-3/CD50 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 189-2G9

Product Details anti-ICAM-3/CD50 Antibody

Purpose: anti-CD50 mouse monoclonal, 189-2G9, purified

Purification: affinity chromatography

Immunogen: stimulated human leucocytes

Isotype: IgG2b kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Alternative Name: CD50

Background: 189-2G9 reacts with the cellular adhesion molecule CD50 (ICAM-3), a single chain polypeptide with a MW of 120 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PI-anchored. 186-2G9 has been clustered at the VIth GLDA International Workshop. 186-2D9 recognized the D1 domain of human CD50 and blocks binding of CD11a (α L-integrin) to CD50. Positive control: HL-60 or THP-1 cells. Lymph node and tonsil.