IFNA2 antibody

Catalog No. ABIN7865671

The Mouse Monoclonal anti-IFNA2 antibody (Clone N27) (ABIN7865671) specifically detects IFNA2 in ELISA, WB and IHC. The antibody is reactive with Human samples.

Quick Overview for IFNA2 antibody (ABIN7865671)

Target: IFNA2 (Interferon, alpha 2 (IFNA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IFNA2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Clone: N27

Product Details anti-IFNA2 Antibody

Purpose: anti-Interferon α 2 mouse monoclonal, N27, purified

Purification: affinity chromatography

Immunogen: E.coli derived recombinant human IFNalpha2c

Isotype: IgG1 kappa

Application Details

Application Notes:

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for IFNA2

Target: IFNA2 (Interferon, alpha 2 (IFNA2))

Alternative Name: Interferon alpha 2

Background: The alpha interferons are involved in virus resistance in target cells for these viruses. They are known to block cell proliferation and to regulate MHC class I antigen expression. The IFNα family has over 20 genes and pseudogenes in two families (I and II), one with a mature length of 166aa and one of 172aa. Cells producing IFNα are lymphocytes, monocytes, macrophages and cell lines such as Namalwa and KGI. Bioassays for IFNα include cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on their target cells. A number of receptors for IFNα are now known and seem to be expressed on most cell types. N27 is specific for human IFNα2 and does not cross react with human IFNα1. N27 reacts with linear peptide 43aa-53aa, placing the epitope outside the immunodominant regions I and II. Positive control: Human IFNα2, Namalwa and KGI cells. Pair: N39

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Hepatitis C