kappa Light Chain antibody

Catalog No. ABIN7865676

The Mouse Monoclonal anti-kappa Light Chain antibody is suitable to detect kappa Light Chain in samples from Human. It has been validated for FACS, IHC, IF, ICC and ELISA.

Quick Overview for kappa Light Chain antibody (ABIN7865676)

Target: kappa Light Chain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This kappa Light Chain antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA

Clone: EBS-huKappa

Product Details anti-kappa Light Chain Antibody

Purpose: anti-Kappa-Light-Chain mouse monoclonal, EBS-huKappa, purified

Cross-Reactivity (Details): negative with rat

Purification: affinity chromatography

Immunogen: B lymphoma cells

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100 - 1:200 (0.5 - 1.0 μg/mL)

FACS_dilution: 0.5 - 1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50 - 1:100 (1 - 2 μg/mL, microwave treatment recommended)

IHC-frozen_dilution: 1:50 - 1:100 (1 - 2 μg/mL)

elisa_dilution: 1:1 - 1:100,000 (0.001 - 100 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0,02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for kappa Light Chain

Target: kappa Light Chain

Alternative Name: Kappa-Light-Chain

Background: EBS-huKappa reacts with kappa light chain In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicate tha the infiltrate is malignant. Positive control: 293T, Raji or hPBL cells, tonsil or spleen.