Keratin 10/13 antibody

Catalog No. ABIN7865677

The Mouse Monoclonal anti-Keratin 10/13 antibody (Clone EBS-IF-003) (ABIN7865677) specifically detects Keratin 10/13 in WB, FACS, IF, IHC and ICC. The antibody is reactive with Human, Cat and Dog samples.

Quick Overview for Keratin 10/13 antibody (ABIN7865677)

Target: Keratin 10/13

Reactivity: Human, Cat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: EBS-IF-003

Product Details

Purpose: anti-Keratin-13+10 mouse monoclonal, EBS-IF-003, purified

Purification: affinity chromatography

Immunogen: cytoskeleton preparation from HeLa cells

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonon vor 15 min, at 4 °C

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details

Target: Keratin 10/13

Alternative Name: Keratin-13+10

Background: EBS-IF-003 reacts with 53 kDa (CK13) and 56.6 kDa (CK10) cytokeratin proteins as indicated by immunoblotting. On formalin-fixed, paraffin-embedded tissue sections EBS-IF-003 recognizes only CK13. On cryostat sections EBS-IF-003 serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas. In paraffin sections EBS-IF-003 does not stain CK10 positive, CK13 negative epithelia, as for example epidermis. Positive control: A431, HeLa, MCF7 cells. Tonsil, esophageal or bladder carcinoma.