KRT14 antibody (C-Term)

Catalog No. ABIN7865678

This Mouse Monoclonal antibody specifically detects KRT14 in IHC, WB, IF, FACS and ICC. It exhibits reactivity toward Human, Rat, Pig and Cat.

Quick Overview for KRT14 antibody (C-Term) (ABIN7865678)

Target: KRT14 (Keratin 14 (KRT14))

Reactivity: Human, Rat, Pig, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: EBS-IF-004

Product Details anti-KRT14 Antibody

Binding Specificity: AA 459-472, C-Term

Purpose: anti-Keratin 14 mouse monoclonal, EBS-IF-004, purified

Purification: affinity chromatography

Immunogen: AA 459-472 at the carboxyterminal end of human cytokeratin 14, conjugated to KLH

Isotype: IgM kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Keratin 14

Background: EBS-IF-004 reacts specifically with keratin 14 (50 kDa) in immunoblotting. In tissue sections EBS-IF-004 is positive with basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas. Positive control: Skin.