MFGE8 antibody

Catalog No. ABIN7865692

The Mouse Monoclonal anti-MFGE8 antibody (Clone MFG-06) (ABIN7865692) specifically detects MFGE8 in WB, IHC, FACS, ICC and IF. The antibody is reactive with Human and Rat samples.

Quick Overview for MFGE8 antibody (ABIN7865692)

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MFGE8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: MFG-06

Product Details anti-MFGE8 Antibody

Purpose: anti-MFG-E8/ Lactadherin/p47 mouse monoclonal, MFG-06, purified

Purification: affinity chromatography

Immunogen: milk fat globule membrane preparation derived from human breast cancer cell line PMC42.

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Alternative Name: MFG-E8/ Lactadherin/p47

Background: MFG-06 reacts with a glycoprotein of 40-45 kDa, known as Milk fat globule-EGF factor 8 protein (MFG-E8), lactadherin, p47 or milk fat globule 1 antigen. It is present on normal epithelial cells in various organs and considered a differentiation marker in carcinomas. It contains one EGF-like domain and 2 F5/8 type C domains. Functioning as a specific ligand for Integrin β5 and Integrin β3, MFG-E8 is thought to be involved in gamete interactions and cell attachment, possibly playing a role in fertilization and apoptosis. Additionally, MFG-E8 binds to rotavirus and inhibits its replication, thereby protecting the cell from viral infection. Overexpression of MFG-E8 is associated with breast cancer, suggesting that MFG-E8 may be related to tumorigenesis or progression. Among testicular carcinomas, seminomas are negative. Positive control: Normal breast or breast carcinoma.

Pathways: SARS-CoV-2 Protein Interactome