MUC2 antibody

Catalog No. ABIN7865698

The Mouse Monoclonal anti-MUC2 antibody (Clone EBS-T-233) (ABIN7865698) specifically detects MUC2 in IHC, WB, IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for MUC2 antibody (ABIN7865698)

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: EBS-T-233

Product Details anti-MUC2 Antibody

Purpose: anti-MUC2 mouse monoclonal, EBS-T-233, purified

Purification: affinity chromatography

Immunogen: synthetic human MUC2 peptide (VNTR region)

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM Tris with 1 mM EDTA, pH 9.0, recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for MUC2

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Alternative Name: MUC2

Background: EBS-T-233 reacts with GTQTP in the VNTR domain of human MUC2 (520 kDa). MUC2 is specifically expressed in goblet cells of the small intestine and colon and about 65 % of colonic carcinomas and about 40 % of gastric carcinomas are positive. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary. Positive control: LS174T cells (flow cytometry, Western blot), small intestine.