MLH1 antibody

Catalog No. ABIN7865702

This Mouse Monoclonal antibody specifically detects MLH1 in IHC. It exhibits reactivity toward Human.

Quick Overview for MLH1 antibody (ABIN7865702)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: IHC409

Product Details anti-MLH1 Antibody

Purpose: anti-MLH1 mouse monoclonal, IHC409, purified

Purification: affinity chromatography

Immunogen: Recombinant Human MLH1

Isotype: IgG2a

Application Details

Application Notes: Optional[ICC/IF_dilution]: 1:50-1:200 (microwave treatment recommended)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Tris pH 7.3-7.7 with 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1

Background: MutL Homolog 1 (MLH1) is a protein involved in the mismatch-repair pathway. This protein is commonly associated with hereditary non-polyposis colorectal cancer, as the MLH1 gene is frequently mutated in patients with this cancer. Studies have shown MLH1 to be deficient in a high percentage of patients with microsatellite instability, as well as endometrial and ovarian cancers. Use of Anti-MLH1 is optimized when paired in an IHC panel with MSH6, MSH2, and PMS2. Anti-MLH1 is useful in the detection of MLH1 in a number of normal and neoplastic tissues, and for identifying a loss of MLH1 in tumours that are microsatellite-unstable.

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response