Proliferation Marker antibody

Catalog No. ABIN7865722

The Mouse Monoclonal anti-Proliferation Marker antibody (Clone IPO-38) (ABIN7865722) specifically detects Proliferation Marker in IHC, WB, ELISA and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Proliferation Marker antibody (ABIN7865722)

Target: Proliferation Marker

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: IPO-38

Product Details

Purpose: anti-Proliferation Marker mouse monoclonal, IPO-38, purified

Purification: affinity chromatography

Immunogen: spleen cells of a patient with hairy cell leukemia

Isotype: IgM kappa

Application Details

Application Notes:

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details

Target: Proliferation Marker

Background: IPO-38 reacts with a 12-14 kDa protein, as found in Western blots of Raji cells, and appears in the mitotic cycle earlier than Ki-67. Lymphocytes, induced to early G1 phase by 12h exposure to PHA, will become positive while non-stimulated lymphocytes remain negative. Mononuclear cells and granulocytes of healthy donors are negative, while various forms of leukemia and lymphoma including Hodgkin’s disease are positive for IPO-38, as are many solid tumors such as some breast, gastric and colonic cancers for which it may serve as tumor progression marker. Positive control: Raji cells.