TGFA antibody

Catalog No. ABIN7865750

This Mouse Monoclonal antibody specifically detects TGFA in IHC, FACS and ELISA. It exhibits reactivity toward Human, Rabbit and Zebrafish (Danio rerio).

Quick Overview for TGFA antibody (ABIN7865750)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human, Rabbit, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Clone: 1E8-G6

Product Details anti-TGFA Antibody

Purpose: anti-TGF-alpha mouse monoclonal, 1E8-G6, purified

Purification: affinity chromatography

Immunogen: synthetic TGF-alpha 34-43 decapeptide linked carrier

Isotype: IgM kappa

Application Details

Application Notes:

FACS_dilution: 0.5-1,0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF-alpha

Background: 1E8-G6 reacts with TGF-alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. 1E8-G6 is completely blocked by the peptide used for immunization. TGF-alpha (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. In sections both cytoplasma and cell membranes are stained. Positive control: Jurkat or Ramos cells. testicular carcinoma, breast cancer.

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway