VCAM1 antibody

Catalog No. ABIN7865753

This Mouse Monoclonal antibody specifically detects VCAM1 in FACS, IHC, ICC, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for VCAM1 antibody (ABIN7865753)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA, Immunofluorescence (IF)

Clone: B-K9

Product Details anti-VCAM1 Antibody

Purpose: anti-CD106 mouse monoclonal, B-K9, purified

Purification: affinity chromatography

Immunogen: activated umbilical cord vein endothelial cells (HUVEC)

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: CD106

Background: CD106 is a protein of 110 kDa, also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100. CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. In addition, epithelial cells and cancers cells can be positive, like in kidney and prostate cancer. CD106 serves as a ligand for leukocytes integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. B-K9 inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells. Positive control: Human placenta or tonsil, kidney, some prostate cancers.

Pathways: Carbohydrate Homeostasis