VEGF antibody

Catalog No. ABIN7865754

The Mouse Monoclonal anti-VEGF antibody is suitable to detect VEGF in samples from Human, Mouse, Rat, Dog and Rabbit. It has been validated for IHC, IF, FACS and ICC.

Quick Overview for VEGF antibody (ABIN7865754)

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Reactivity: Human, Mouse, Rat, Dog, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This VEGF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: VEGF-21

Product Details anti-VEGF Antibody

Purpose: anti-Vascular Endothelial Growth Factor (VEGF) mouse monoclonal, VEGF-21, purified

Purification: affinity chromatography

Immunogen: human VEGF189 recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml

IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for VEGF

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Background: VEGF-21 reacts with Vascular Endothelial Growth Factor, also known as Vascular Permeability Factor (VEGF/ VPF) and is the key mediator of angiogenesis. The MWs are 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. In addition to endothelial cells, VEGF and VEGF receptors are expressed on numerous non-endothelial cells including tumor cells. Positive control: Astrocytomas, breast or ovarian carcinomas.