CD2 antibody

Catalog No. ABIN7866122

The Mouse Monoclonal anti-CD2 antibody is suitable to detect CD2 in samples from Human. It has been validated for FACS.

Quick Overview for CD2 antibody (ABIN7866122)

Target: CD2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD2 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: T6-3

Product Details anti-CD2 Antibody

Purpose: Mouse anti Human CD2

Characteristics: Mouse anti human CD2 can be used for identification of human T cells and a subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.

Purification: Protein A/G Chromatography

Immunogen: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by a mixed lymphocyte culture.

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: PBMC: Add 10 μL of antibody to 1 million PBMCs in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 μL of antibody to 100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for immunofluorescence analysis with a flow cytometer or microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CD2

Target: CD2

Alternative Name: CD2

Background: T-cell surface antigen CD2, CD2 cytoplasmic domain-binding protein

UniProt: P06729