Recombinant CD56 antibody

Catalog No. ABIN7866125

The Mouse Monoclonal anti-CD56 antibody is suitable to detect CD56 in samples from Human. It has been validated for FACS, IHC (p), ICC and IHC (fro).

Quick Overview for Recombinant CD56 antibody (ABIN7866125)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD56 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: C5-9 E1 (derived from clone C5-9)

Product Details anti-CD56 Antibody

Purpose: Recombinant Mouse anti Human CD56

Characteristics: NCAM / CD56, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM can be found in central and peripheral nerve cells, neuroendocrine tissues and at the surface of NK-cells. Also, NCAM is present in malignancies derived from these tissues and cells.

Purification: Protein A/G Chromatography

Immunogen: Mouse monoclonal antibody 0566P is a recombinant CD56 monoclonal antibody produced in mammalian HEK293 cells. It is produced by cloning the antibody sequence of the mouse anti CD56 hybridoma C5.9, which was derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with an extract from the KG1a cell line.

Isotype: IgG2b

Application Details

Application Notes: 0566P is suitable for flow cytometry, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedd tissue sections. Optimal antibody dilution should be determined by titration, recommended range is 1:200 - 1:500 for immunohistochemistry with fluorochrome conjugated secondary antibodies or with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 100 µL 1 mg/mL ProtA purified recombinant monoclonal antibody in PBS containing 0.09 % sodium azide. The purity of the antibody is >95 % as determined by SDS-Polyacrylamide gel electrophoresis. The endotoxin level is < 1EU/mg as determined by the LAL chromogenic endotoxin assay.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: CD56

Background: CD56/Neural Cell Adhesion Molecule/NCAM