Integrin beta 3 antibody

Catalog No. ABIN7866126

The Mouse Monoclonal anti-Integrin beta 3 antibody (Clone 43-PL) (ABIN7866126) specifically detects Integrin beta 3 in FACS. The antibody is reactive with Human samples.

Quick Overview for Integrin beta 3 antibody (ABIN7866126)

Target: Integrin beta 3 (ITGB3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 3 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 43-PL

Product Details anti-Integrin beta 3 Antibody

Purpose: Mouse anti Human CD61

Characteristics: CD61 recognizes an Mr 110-kdalton ( kDa) protein, also known as gpIIIa, the common beta-subunit (integrin beta 3-chain) of the gpIIb/IIIa complex and the vitronectin receptor (VNR). The gpIIb/IIIa complex and the VNR are integrins, ie, a/b-heterodimeric glycoprotein complexes that are involved in cell adhesion. With the CD41 antigen (gpIIb or aIIb), the CD61 antigen forms the gpIIb/IIIa complex, which acts as a receptor for fibrinogen, von Willebrand factor (vWf), fibronectin and vitronectin on activated platelets. With the CD51 antigen (VNR a-chain or av), the CD61 antigen forms the VNR, which mediates activation-independent cell adhesion to vitronectin, vWf, fibrinogen, and thrombospondin. The CD61 antigen is found on all normal resting and activated platelets. The CD61 antigen is also found on endothelial cells, megakaryocytes, on some myeloid, erythroid and T-lymphoid leukemic cell lines.

Purification: Protein A/G Chromatography

Immunogen: Derived from the hybridization of mouse X63.Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with purified platelet membrane glycoproteins

Isotype: IgG1

Application Details

Application Notes: PBMC: Add10 μL of MAB/10^6 PBMC in 100 μL PBS. Mix gently and incubate for 15 minutes at 2° to 8 °C. Wash twice with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 μL of MAB/100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20 °C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Integrin beta 3

Target: Integrin beta 3 (ITGB3)

Alternative Name: CD61

Background: CD61

UniProt: P05106

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex