S100A8/A9 Complex (Calprotectin) antibody (AA 1-100)

Catalog No. ABIN7867818

The Mouse Monoclonal anti-S100A8/A9 Complex (Calprotectin) antibody is suitable to detect S100A8/A9 Complex (Calprotectin) in samples from Human. It has been validated for IHC (p).

Quick Overview for S100A8/A9 Complex (Calprotectin) antibody (AA 1-100) (ABIN7867818)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: S100A9-7551

Product Details anti-S100A8/A9 Complex (Calprotectin) Antibody

Binding Specificity: AA 1-100

Purpose: Calprotectin Antibody / S100A9 / MRP14

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 1-100) from the human protein was used as the immunogen for the Calprotectin antibody.

Isotype: IgG2, kappa

Application Details

Application Notes: Optimal dilution of the Calprotectin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Calprotectin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Alternative Name: Calprotectin

Background: This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer s cells of the liver and Langerhan s cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

UniProt: P06702

Pathways: S100 Proteins