Recombinant E2F4 antibody (AA 1-100)

Catalog No. ABIN7867851

The Rabbit Monoclonal anti-E2F4 antibody is suitable to detect E2F4 in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant E2F4 antibody (AA 1-100) (ABIN7867851)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This E2F4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: E2F4-8811R

Product Details anti-E2F4 Antibody

Binding Specificity: AA 1-100

Purpose: E2F4 Antibody (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 1-100) from the human protein was used as the immunogen for the E2F4 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the E2F4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Aliquot the E2F4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Alternative Name: E2F4

Background: The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an active E2F transcriptional regulatory complex.

UniProt: Q16254

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size