MYOD1 antibody (AA 1-120)

Catalog No. ABIN7868018

This Rabbit Polyclonal antibody specifically detects MYOD1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MYOD1 antibody (AA 1-120) (ABIN7868018)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MYOD1 Antibody

Binding Specificity: AA 1-120

Purpose: MYOD1 Antibody / Myoblast determination protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MYOD1 recombinant protein (Position: M1-R120) was used as the immunogen for the MYOD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MYOD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MYOD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MYOD1

Background: MYOD1 antibody detects Myoblast determination protein 1, a master transcription factor that controls skeletal muscle differentiation. The UniProt recommended name is Myoblast determination protein 1 (MYOD1). This basic helix-loop-helix (bHLH) transcription factor binds DNA at E-box motifs to activate muscle-specific gene expression, converting multipotent mesodermal cells into myoblasts.

Functionally, MYOD1 antibody identifies a 320-amino-acid nuclear protein that dimerizes with E proteins to regulate the transcription of myogenic regulatory genes such as MYOG and MYF5. MYOD1 acts as a pioneer factor, remodeling chromatin to establish muscle-specific transcriptional programs. It is indispensable for myogenic lineage commitment and muscle regeneration following injury.

The MYOD1 gene is located on chromosome 11p15.1 and is expressed in skeletal muscle progenitor cells and differentiating myoblasts. MYOD1 operates in coordination with MEF2 family transcription factors to control muscle fiber formation and repair. During development, its expression defines the onset of the myogenic program.

Pathologically, MYOD1 dysregulation is associated with muscle developmental disorders and certain sarcomas. Mutations in MYOD1 have been identified in rhabdomyosarcoma, where aberrant transcriptional activation promotes tumorigenesis. Research using MYOD1 antibody supports studies in muscle development, differentiation, and cancer biology.

MYOD1 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect myogenic transcription factors. NSJ Bioreagents provides MYOD1 antibody reagents optimized for skeletal muscle biology, stem cell differentiation, and gene regulation research.

Structurally, Myoblast determination protein 1 contains a bHLH domain that mediates DNA binding and dimerization. Its N-terminal activation domain recruits chromatin remodeling complexes to activate transcription. This antibody facilitates detailed examination of MYOD1's regulatory network in muscle cell fate determination.

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development