APAF1 antibody (AA 1-1248)

Catalog No. ABIN7868026

The Rabbit Polyclonal anti-APAF1 antibody (ABIN7868026) specifically detects APAF1 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for APAF1 antibody (AA 1-1248) (ABIN7868026)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-APAF1 Antibody

Binding Specificity: AA 1-1248

Purpose: Apoptotic protease-activating factor 1 Antibody / APAF1

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids M1-E1248) was used as the immunogen for the Apoptotic protease-activating factor 1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Apoptotic protease-activating factor 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Apoptotic protease-activating factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apoptotic protease-activating factor 1

Background: Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23. It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis. This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9. Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death. Additionally, APAF1 has been shown to interact with NLRP1, Caspase-9, APIP, BCL2-like 1 and HSPA4.

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity