Vinculin antibody (AA 1-149)

Catalog No. ABIN7868181

The Rabbit Polyclonal anti-Vinculin antibody is suitable to detect Vinculin in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, ELISA, IF, IHC (p) and FACS.

Quick Overview for Vinculin antibody (AA 1-149) (ABIN7868181)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-Vinculin Antibody

Binding Specificity: AA 1-149

Purpose: Vinculin Antibody / VCL

Purification: Affinity purified

Immunogen: Recombinant human protein (amino acids M1-E149) was used as the immunogen for the Vinculin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Vinculin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Vinculin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. It is mapped to 10q22.2. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2