RPL24 antibody (AA 1-157)

Catalog No. ABIN7868220

The Rabbit Polyclonal anti-RPL24 antibody is suitable to detect RPL24 in samples from Human, Mouse and Rat. It has been validated for WB, IF, ELISA and ICC.

Quick Overview for RPL24 antibody (AA 1-157) (ABIN7868220)

Target: RPL24 (Ribosomal Protein L24 (RPL24))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL24 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Product Details anti-RPL24 Antibody

Binding Specificity: AA 1-157

Purpose: RPL24 Antibody / 60S ribosomal protein L24

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RPL24 recombinant protein (Position: M1-R157) was used as the immunogen for the RPL24 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RPL24 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RPL24 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RPL24

Target: RPL24 (Ribosomal Protein L24 (RPL24))

Alternative Name: RPL24

Background: RPL24 antibody detects 60S ribosomal protein L24, a structural component of the large ribosomal subunit essential for protein synthesis. Encoded by the RPL24 gene on chromosome 3p21.3, this ribosomal protein contributes to ribosome assembly, rRNA processing, and translation elongation. RPL24 participates in maintaining translational fidelity and efficiency, influencing cell growth, proliferation, and metabolic control.

Structurally, RPL24 is a 157-amino-acid protein of approximately 18 kilodaltons that forms part of the ribosomal 60S subunit. It is highly conserved across species and interacts with ribosomal RNA and neighboring proteins to stabilize the ribosomal architecture. RPL24 localizes predominantly to the cytoplasm and nucleolus, reflecting its dual role in ribosome biogenesis and cytoplasmic translation.

The RPL24 antibody is widely used in molecular biology, cancer research, and translational control studies to analyze ribosome composition, protein synthesis rates, and growth regulation. Western blot analysis identifies an 18 kilodalton band corresponding to RPL24, while immunofluorescence shows strong cytoplasmic and nucleolar localization. This antibody supports research examining how ribosomal proteins coordinate mRNA translation with cellular metabolism and proliferation.

Functionally, RPL24 promotes ribosome maturation by assisting in 28S rRNA folding and subunit joining. Reduced RPL24 expression impairs translation efficiency and causes growth defects, while overexpression supports rapid proliferation in tumor cells. Mutations in ribosomal proteins including RPL24 are associated with developmental syndromes and cancers characterized by deregulated protein synthesis. The RPL24 antibody provides a critical tool for studying ribosome biogenesis, translational regulation, and ribosomal stress responses. NSJ Bioreagents validates this antibody for its applications, ensuring accurate detection for studies of translation and growth control.

UniProt: P83731

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly