MED28 antibody (AA 1-178)

Catalog No. ABIN7868262

The Rabbit Polyclonal anti-MED28 antibody is suitable to detect MED28 in samples from Human. It has been validated for ELISA, WB, FACS and IP.

Quick Overview for MED28 antibody (AA 1-178) (ABIN7868262)

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED28 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-MED28 Antibody

Binding Specificity: AA 1-178

Purpose: MED28 Antibody / Mediator complex subunit 28

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MED28 recombinant protein (Position: M1-T178) was used as the immunogen for the MED28 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MED28 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MED28 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MED28

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Alternative Name: MED28

Background: MED28 antibody detects Mediator complex subunit 28, a component of the Mediator coactivator complex that bridges transcription factors and RNA polymerase II during transcription initiation. MED28 plays essential roles in gene expression, cytoskeletal organization, and signal transduction. The MED28 antibody is widely used in transcriptional regulation, cancer, and developmental biology research to study Mediator function and signaling integration.

MED28 is encoded by the MED28 gene located on human chromosome 4q27. The protein is approximately 178 amino acids long and localizes to both the nucleus and plasma membrane. MED28 interacts with other Mediator complex subunits as well as cytoskeletal proteins, suggesting dual functions in transcriptional control and cell structure regulation. Its nuclear role involves recruitment of RNA polymerase II to promoters and modulation of gene-specific transcriptional activation.

The MED28 antibody detects a 22 kilodalton band by western blot and shows nuclear and cortical cytoplasmic staining. MED28 acts as a scaffold linking the cytoskeleton to transcriptional machinery, coordinating extracellular signals with gene expression. It associates with receptor tyrosine kinases and small GTPases, influencing actin organization and cell migration.

Functionally, MED28 contributes to development and differentiation by regulating transcription factors involved in lineage commitment. Dysregulation of MED28 expression has been linked to various cancers, including breast and gastric carcinoma, where it enhances proliferation and motility. MED28 also participates in smooth muscle differentiation and cardiovascular development, underscoring its diverse physiological roles.

Through its integration of signaling and transcriptional regulation, MED28 serves as a nexus between cellular structure and gene expression. NSJ Bioreagents provides a validated MED28 antibody optimized for its applications, supporting research into Mediator complex biology, cytoskeletal signaling, and transcriptional control.

UniProt: Q9H204

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation