IL-18 antibody (AA 1-193)

Catalog No. ABIN7868336

This Mouse Monoclonal antibody specifically detects IL-18 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for IL-18 antibody (AA 1-193) (ABIN7868336)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IL18-4623

Product Details anti-IL-18 Antibody

Binding Specificity: AA 1-193

Purpose: Interleukin 18 Antibody / IL-18

Purification: Protein A/G affinity

Immunogen: A recombinant fragment (within amino acids 1-193) of human IL-18 protein was used as the immunogen for the Interleukin 18 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the Interleukin 18 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Interleukin 18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: Interleukin 18

Background: Four structurally related IL-1 receptor ligands have been described. These include three agonists designated IL-1a, IL-1b and IL-1g/IL-18 and a specific receptor antagonist, IL-1Ra. IL-1a and IL-1b play critical roles in the regulation of the immune response and inflammation, serving as activators of T and B lymphocytes and NK (natural killer) cells. IL-18 (also referred to as IL-1g) has been shown to augment the secretion of IFN-g from T lymphocytes and increase NK cell activity in spleen cells. IL-18 exhibits 19 % and 12 % identity with IL-1a and IL-1b respectively over the 12 beta-strands of the beta-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1b pro-domain which must be cleaved by the serine protease ICE for optimal secretion and biological activity. Originally described as IGIF (IFN-g-inducing factor), IL-18 is induced by mouse liver subsequent to challenge with lipopolysaccharide (LPS).

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome