TFAM antibody (AA 1-200)

Catalog No. ABIN7868775

The Mouse Monoclonal anti-TFAM antibody is suitable to detect TFAM in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for TFAM antibody (AA 1-200) (ABIN7868775)

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TFAM-9713

Product Details anti-TFAM Antibody

Binding Specificity: AA 1-200

Purpose: mtTFA Antibody | Mitochondrial transcription factor 1 / TFAM

Purification: Protein A/G affinity

Immunogen: A portion of amino acids 1-200 from human Mitochondrial transcription factor 1 protein was used as the immunogen for the mtTFA antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the mtTFA antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the mtTFA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Alternative Name: mtTFA

Background: MtTFA (mitochondrial transcription factor A), also known as mtTF1, TFAM, TCF6 (for transcription factor 6-like1), TCF6L2 and tsHMG, is a nuclear-encoded gene product that is imported into the mitochondria. mtTFA is required for many aspects of mitochondrial biogenesis including the replication and transcription of mitochondrial DNA (mtDNA). In mouse, testis-specific mtTFA is missing the mitochondria targeting sequence and is present in the nucleus rather than the mitochondria. This form of mtTFA is located primarily in the nuclei of elongated spermatids and may be involved in the regulation of gene expression of the haploid male genome. During mouse and human spermatogenesis there is a reduction of mtTFA protein levels and a reduction in mtDNA copy number. These features may provide one of the mechanisms by which paternal mtDNA transmission is prevented. mtTFA has been associated with mitochondrial disorder in humans characterized by ocular myopathy, exercise intolerance and muscle wasting.

UniProt: Q00059

Pathways: Chromatin Binding