RPL14 antibody (AA 1-207)

Catalog No. ABIN7868817

The Rabbit Polyclonal anti-RPL14 antibody is suitable to detect RPL14 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF, FACS and ICC.

Quick Overview for RPL14 antibody (AA 1-207) (ABIN7868817)

Target: RPL14 (Ribosomal Protein L14 (RPL14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-RPL14 Antibody

Binding Specificity: AA 1-207

Purpose: RPL14 Antibody / 60S ribosomal protein L14

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RPL14 recombinant protein (Position: M1-A207) was used as the immunogen for the RPL14 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RPL14 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RPL14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RPL14

Target: RPL14 (Ribosomal Protein L14 (RPL14))

Alternative Name: RPL14

Background: RPL14 antibody detects 60S ribosomal protein L14, a structural component of the large ribosomal subunit involved in translation and ribosome assembly. RPL14 is one of approximately 80 ribosomal proteins that form the functional ribosome, catalyzing peptide bond formation and mRNA decoding. The RPL14 antibody is used in studies of ribosome biogenesis, translational control, and nucleolar organization.

RPL14 is encoded by the RPL14 gene located on human chromosome 3p21.3. The protein is about 23 kilodaltons and localizes predominantly in the cytoplasm and nucleolus. RPL14 integrates into the 60S ribosomal subunit near the peptidyl transferase center, stabilizing interactions between rRNA and ribosomal proteins during translation. Through these roles, RPL14 ensures accuracy and efficiency of protein synthesis.

The RPL14 antibody detects a 23 kilodalton band on western blot and demonstrates both cytoplasmic and nucleolar staining by immunofluorescence. RPL14 expression correlates with proliferative activity, and its upregulation is often seen in rapidly dividing cells or tumors. Inhibition of RPL14 synthesis activates ribosomal stress pathways, leading to p53 stabilization and growth arrest. Mutations or pseudogene-derived transcripts of RPL14 may contribute to tumorigenesis or ribosomopathies characterized by anemia and developmental defects.

Beyond translation, RPL14 participates in extra-ribosomal functions, including regulation of apoptosis and cell cycle progression. Under stress conditions, RPL14 can interact with transcription factors or signaling proteins, modulating gene expression independent of ribosome assembly. Proteomic studies have also detected RPL14 in mitochondrial ribosome fractions, suggesting roles in organelle-specific translation.

Because ribosomal proteins are frequently deregulated in cancer, RPL14 serves as a biomarker for tumor cell proliferation and ribosome biogenesis activity. Elevated expression has been observed in colorectal, breast, and ovarian cancers, correlating with poor prognosis. Conversely, knockdown of RPL14 reduces protein synthesis and inhibits tumor growth. NSJ Bioreagents provides a validated RPL14 antibody optimized for western blot, immunocytochemistry, and ribosomal complex analysis, supporting exploration of translation dynamics, nucleolar function, and cancer-related ribosome biology.

UniProt: P50914