SURF6 antibody (AA 1-216)

Catalog No. ABIN7868836

The Rabbit Polyclonal anti-SURF6 antibody (ABIN7868836) specifically detects SURF6 in WB, ELISA, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for SURF6 antibody (AA 1-216) (ABIN7868836)

Target: SURF6 (Surfeit 6 (SURF6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SURF6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-SURF6 Antibody

Binding Specificity: AA 1-216

Purpose: SURF6 Antibody / Surfeit locus protein 6

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SURF6 recombinant protein (Position: M1-R216) was used as the immunogen for the SURF6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SURF6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SURF6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SURF6

Target: SURF6 (Surfeit 6 (SURF6))

Alternative Name: SURF6

Background: SURF6 antibody detects Surfeit locus protein 6, a nucleolar protein involved in ribosome biogenesis and pre-rRNA processing. The UniProt recommended name is Surfeit locus protein 6 (SURF6). This evolutionarily conserved nucleolar factor functions as a scaffold for ribonucleoprotein complex assembly during early steps of ribosome formation.

Functionally, SURF6 antibody identifies a 360-amino-acid protein localized to the granular component of the nucleolus, where it associates with pre-rRNA transcripts and ribosomal assembly intermediates. SURF6 interacts with fibrillarin and nucleolin, facilitating pre-rRNA maturation and 60S ribosomal subunit formation. Its RNA-binding capacity and nucleolar localization signal make it an essential factor for ribosome production and nucleolar organization.

The SURF6 gene is located on chromosome 9q34.2 and is expressed ubiquitously, with high levels in proliferating and metabolically active cells. Expression correlates with cellular growth rate, reflecting its role in ribosome synthesis and protein translation capacity.

Pathologically, dysregulation of SURF6 expression has been linked to oncogenesis and stress response modulation. Overexpression is observed in various cancers and is associated with increased ribosome biogenesis and cell proliferation. Conversely, depletion of SURF6 triggers nucleolar stress, p53 activation, and growth arrest. Research using SURF6 antibody supports studies in ribosome assembly, nucleolar dynamics, and cancer biology.

SURF6 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect nucleolar proteins. NSJ Bioreagents provides SURF6 antibody reagents optimized for studies in ribosomal biogenesis, nucleolar organization, and cellular stress response.

Structurally, Surfeit locus protein 6 contains arginine-rich motifs that mediate RNA binding and multiple acidic stretches that promote protein-protein interactions within the nucleolus. Its modular domains contribute to the architecture of the ribosomal assembly machinery. This antibody facilitates exploration of SURF6's function in nucleolar integrity and ribosome maturation.

UniProt: O75683

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly