SOD2 antibody (AA 1-224)

Catalog No. ABIN7868858

This Rabbit Polyclonal antibody specifically detects SOD2 in WB. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for SOD2 antibody (AA 1-224) (ABIN7868858)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SOD2 Antibody

Binding Specificity: AA 1-224

Purpose: Zebrafish Sod2 Antibody / Superoxide dismutase 2

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Sod2 recombinant protein (amino acids M1-K224) was used as the immunogen for the Zebrafish Sod2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Sod2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Sod2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: Sod2

Background: Zebrafish Sod2, also known as superoxide dismutase 2, is a manganese containing antioxidant enzyme located in the mitochondrial matrix. It catalyzes the dismutation of superoxide radicals into hydrogen peroxide and oxygen, which is a critical step in defending cells against oxidative stress generated during mitochondrial respiration. Sod2 is essential for maintaining mitochondrial integrity, reducing oxidative damage to proteins, lipids, and DNA, and ensuring proper cellular metabolism and survival.

Zebrafish Sod2 is an ortholog of the human SOD2 protein, with strong sequence and functional conservation. As in humans, Sod2 in zebrafish plays a vital role in protecting highly metabolic tissues such as the brain, heart, and muscles from oxidative damage. Mutations or reduced activity of Sod2 can lead to mitochondrial dysfunction and oxidative stress, contributing to developmental abnormalities and degenerative diseases.

Currently, there are no confirmed isoforms of zebrafish Sod2. It is broadly expressed across tissues but is particularly abundant in cells with high energy demands. Zebrafish models deficient in Sod2 have been used to investigate the consequences of oxidative imbalance and to explore therapeutic approaches aimed at reducing mitochondrial stress.

Antibodies targeting zebrafish Sod2 protein are important tools for studying mitochondrial antioxidant defense systems, oxidative signaling pathways, and stress responses. These antibodies can be utilized in western blot, immunohistochemistry, immunofluorescence, and ELISA to monitor Sod2 levels and localization in zebrafish tissues.

UniProt: Q6P980

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling