PSMA5 antibody (AA 1-241)

Catalog No. ABIN7868906

This Rabbit Polyclonal antibody specifically detects PSMA5 in WB and IHC (p). It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for PSMA5 antibody (AA 1-241) (ABIN7868906)

Target: PSMA5 (Proteasome Subunit alpha 5 (PSMA5))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PSMA5 Antibody

Binding Specificity: AA 1-241

Purpose: Zebrafish Psma5 Antibody / Proteasome subunit alpha type 5

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Psma5 recombinant protein (amino acids M1-I241) was used as the immunogen for the Zebrafish Psma5 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Psma5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Psma5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PSMA5

Target: PSMA5 (Proteasome Subunit alpha 5 (PSMA5))

Alternative Name: Psma5

Background: Psma5 (Proteasome subunit alpha type 5) is a structural component of the 20S core particle of the proteasome, a key intracellular complex responsible for the ATP-independent degradation of ubiquitinated proteins. Psma5 belongs to the alpha subunit family, which forms the two outer rings of the proteasome core. These alpha subunits regulate substrate entry into the proteolytic chamber formed by the beta subunits and are essential for proteasome assembly, stability, and function.

In zebrafish, Psma5 is the ortholog of the human PSMA5 gene. The protein sequence and functional domains are highly conserved between zebrafish and humans, indicating a shared role in proteasome-mediated protein degradation. This evolutionary conservation supports the use of zebrafish as a model organism for studying proteostasis, developmental regulation, and disease mechanisms related to proteasome dysfunction.

Zebrafish Psma5 may have multiple isoforms generated through alternative splicing. These isoforms could allow tissue-specific or developmental stage-specific regulation of proteasome activity, although the canonical isoform is primarily involved in the assembly and function of the standard 20S proteasome.

During zebrafish embryogenesis, Psma5 is broadly expressed in rapidly proliferating and metabolically active tissues, including the developing brain, eye, musculature, and digestive organs. Its expression reflects the universal need for controlled protein degradation in processes such as cell cycle progression, signal transduction, and removal of damaged or misfolded proteins.

In human biology, defects in proteasome components including PSMA5 have been associated with neurodegenerative diseases, cancers, and immune system disorders. Zebrafish Psma5, due to its conserved function, serves as a valuable model for functional studies of the proteasome and for the development of therapeutics targeting the ubiquitin-proteasome system.

UniProt: Q6TGV6

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA