Prohibitin 2 antibody (AA 1-299)

Catalog No. ABIN7869077

This Mouse Monoclonal antibody specifically detects Prohibitin 2 in WB, IHC (p) and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Prohibitin 2 antibody (AA 1-299) (ABIN7869077)

Target: Prohibitin 2 (PHB2)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 2B5

Product Details anti-Prohibitin 2 Antibody

Binding Specificity: AA 1-299

Purpose: Prohibitin 2 Antibody / PHB2

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids M1-K299) was used as the immunogen for the Prohibitin 2 antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the Prohibitin 2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Prohibitin 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Prohibitin 2

Target: Prohibitin 2 (PHB2)

Alternative Name: Prohibitin 2

Background: PHB2 (Prohibitin 2), also called Repressor of Estrogen Receptor Activity (REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al.(1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta(ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling