The Rabbit Polyclonal anti-ETFA antibody is suitable to detect ETFA in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC (p).
ETFA
Reactivity: Human, Mouse, Rat
WB, IHC, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal dilution of the ETFA antibody should be determined by the researcher.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Storage
4 °C,-20 °C
Storage Comment
After reconstitution, the ETFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
The human ETFA gene encodes the Electron-transfer-flavoprotein, alpha subunit, also known as ETF-a. Together with Electron-transfer-flavoprotein, beta subunit, encoded by the 'ETFB' gene, it forms the heterodimeric Electron transfer flavoprotein (ETF). ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.