SKA3 antibody (AA 1-412)

Catalog No. ABIN7869328

The Rabbit Polyclonal anti-SKA3 antibody (ABIN7869328) specifically detects SKA3 in ELISA, WB and IHC. The antibody is reactive with Human samples.

Quick Overview for SKA3 antibody (AA 1-412) (ABIN7869328)

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKA3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SKA3 Antibody

Binding Specificity: AA 1-412

Purpose: SKA3 Antibody / Spindle and kinetochore associated complex subunit 3 / C13orf3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human C13orf3/SKA3 recombinant protein (Position: M1-N412) was used as the immunogen for the SKA3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SKA3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SKA3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SKA3

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Alternative Name: SKA3

Background: SKA3 antibody detects Spindle and kinetochore associated complex subunit 3, a mitotic protein required for chromosome segregation and kinetochore-microtubule attachment. The UniProt recommended name is Spindle and kinetochore associated complex subunit 3 (SKA3). Also known as C13orf3, this protein forms part of the SKA complex, along with SKA1 and SKA2, which stabilizes kinetochore-spindle microtubule interactions during metaphase and anaphase.

Functionally, SKA3 antibody identifies a 412-amino-acid cytoplasmic and kinetochore-localized protein that binds microtubules and interacts with the Ndc80 complex to anchor chromosomes to spindle fibers. The SKA complex is recruited to kinetochores in a microtubule-dependent manner and facilitates the coupling of depolymerizing microtubules to chromosome movement. SKA3 plays a crucial role in maintaining mitotic fidelity and preventing chromosome missegregation. It is also required for silencing the spindle assembly checkpoint once proper attachment is achieved, allowing progression into anaphase.

The SKA3 gene is located on chromosome 13q12.13 and is expressed in proliferating cells, with elevated expression during the G2/M phase of the cell cycle. Its transcription is regulated by E2F family transcription factors, linking SKA3 expression to cell cycle progression and mitotic entry.

Pathologically, dysregulation of SKA3 contributes to chromosomal instability, aneuploidy, and cancer. Overexpression has been observed in breast, liver, and lung cancers, where it promotes uncontrolled proliferation and correlates with poor prognosis. Loss of SKA3 function disrupts kinetochore attachment and induces mitotic arrest. Research using SKA3 antibody supports studies in mitosis, spindle checkpoint control, and cancer biology.

SKA3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect mitotic spindle-associated proteins. NSJ Bioreagents provides SKA3 antibody reagents optimized for research in chromosome segregation, cell division, and tumorigenesis.

Structurally, Spindle and kinetochore associated complex subunit 3 contains coiled-coil domains that mediate oligomerization and interaction with SKA1/SKA2. Its C-terminal region binds directly to microtubules, while its N-terminal domain interacts with kinetochore complexes. This modular organization enables SKA3 to function as a mechanical linker between depolymerizing microtubules and kinetochores. This antibody allows precise analysis of SKA3's role in mitotic regulation and chromosomal stability.

UniProt: Q8IX90