SNAP47 antibody (AA 1-440)

Catalog No. ABIN7869366

The Rabbit Polyclonal anti-SNAP47 antibody is suitable to detect SNAP47 in samples from Human. It has been validated for WB and ELISA.

Quick Overview for SNAP47 antibody (AA 1-440) (ABIN7869366)

Target: SNAP47 (Synaptosomal-Associated Protein, 47kDa (SNAP47))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAP47 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SNAP47 Antibody

Binding Specificity: AA 1-440

Purpose: SNAP47 Antibody / Synaptosomal-associated protein 47

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SNAP47 recombinant protein (Position: M1-D440) was used as the immunogen for the SNAP47 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SNAP47 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SNAP47 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SNAP47

Target: SNAP47 (Synaptosomal-Associated Protein, 47kDa (SNAP47))

Alternative Name: SNAP47

Background: SNAP47 antibody detects Synaptosomal-associated protein 47, encoded by the SNAP47 gene on chromosome 1q32.1. SNAP47 antibody is commonly used to study this SNARE family protein involved in vesicular trafficking and neurotransmitter release. SNAP47 belongs to the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) superfamily, which mediates vesicle fusion with target membranes. Unlike classical SNAP25, SNAP47 is not palmitoylated and has distinct regulatory functions in neuronal and non-neuronal cells. It is expressed in brain, spinal cord, endocrine tissues, and other organs where vesicle trafficking is critical.

Structurally, SNAP47 contains coiled-coil SNARE motifs that form complexes with other SNARE proteins such as Syntaxins and VAMPs. These complexes enable vesicle docking and membrane fusion. SNAP47 lacks the cysteine-rich palmitoylation sites of SNAP25, which influences its membrane association and trafficking dynamics. It localizes to intracellular membranes, including the Golgi apparatus, recycling endosomes, and synaptic vesicles.

Functionally, SNAP47 regulates synaptic vesicle exocytosis and intracellular trafficking. It contributes to neurotransmitter release by participating in alternative SNARE complexes. SNAP47 also functions in recycling of synaptic vesicles and endosomal sorting, impacting synaptic plasticity and neurotransmission efficiency. Knockdown of SNAP47 in neurons results in impaired neurotransmission and defects in vesicle recycling. Researchers employ SNAP47 antibody to study neurotransmission, SNARE complex assembly, and membrane trafficking pathways.

Clinically, SNAP47 dysregulation has been associated with neuropsychiatric disorders such as schizophrenia and autism spectrum disorders, where altered synaptic vesicle cycling contributes to disease mechanisms. Genome studies have linked SNAP47 variants to cognitive traits and neurological conditions. Aberrant expression has also been observed in certain cancers, where vesicle trafficking contributes to tumor progression. NSJ Bioreagents provides SNAP47 antibody to enable studies of synaptic biology, vesicular trafficking, and disease pathogenesis.

Experimentally, SNAP47 antibody is used in western blotting to detect the ~47 kDa protein, in immunofluorescence microscopy to study intracellular localization, and in immunoprecipitation assays to analyze SNARE complex composition. These tools facilitate detailed exploration of vesicle fusion mechanisms in neurons and other cell types.

UniProt: Q5SQN1