Dystrobrevin beta antibody (AA 1-627)

Catalog No. ABIN7869507

The Rabbit Polyclonal anti-Dystrobrevin beta antibody is suitable to detect Dystrobrevin beta in samples from Human, Rat and Mouse. It has been validated for WB, ELISA and FACS.

Quick Overview for Dystrobrevin beta antibody (AA 1-627) (ABIN7869507)

Target: Dystrobrevin beta (DTNB) (Dystrobrevin, beta (DTNB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dystrobrevin beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Dystrobrevin beta Antibody

Binding Specificity: AA 1-627

Purpose: DTNB Antibody / Dystrobrevin beta

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human DTNB recombinant protein (Position: M1-G627) was used as the immunogen for the DTNB antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DTNB antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the DTNB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Dystrobrevin beta

Target: Dystrobrevin beta (DTNB) (Dystrobrevin, beta (DTNB))

Alternative Name: DTNB

Background: DTNB antibody detects Dystrobrevin beta, a cytoplasmic scaffolding protein that associates with dystrophin and syntrophin complexes at the plasma membrane. DTNB participates in maintaining membrane stability, signal transduction, and cytoskeletal anchoring in muscle and neuronal tissues. The DTNB antibody is widely used in muscle biology, neuroscience, and membrane research to study dystrophin-associated complexes and neuromuscular organization.

DTNB is encoded by the DTNB gene located on human chromosome 2p16.2. The protein is approximately 628 amino acids in length and shares structural similarity with dystrobrevin alpha. DTNB localizes to the cytoplasmic face of the sarcolemma and interacts with dystrophin, syntrophins, and other adaptor proteins within the dystrophin-glycoprotein complex (DGC), which links the cytoskeleton to the extracellular matrix.

The DTNB antibody detects a 70 kilodalton protein by western blot and reveals sarcolemmal and cytoplasmic localization under immunofluorescence microscopy. In skeletal muscle, DTNB contributes to stabilization of muscle fibers and transmission of mechanical force. In neurons, it participates in postsynaptic density organization and signal transduction, influencing synaptic plasticity and neurotransmission.

Dysfunction or loss of DTNB disrupts DGC integrity, leading to impaired muscle contractility and signaling abnormalities. Variants in DTNB have been associated with neuromuscular diseases, cognitive deficits, and cardiomyopathy. Through its scaffolding functions, DTNB regulates localization of signaling molecules such as nitric oxide synthase and kinases involved in muscle adaptation.

Because of its role in linking the cytoskeleton to membrane signaling complexes, DTNB provides insights into muscular dystrophy mechanisms and synaptic organization. NSJ Bioreagents provides a validated DTNB antibody optimized for its applications, supporting research into muscle integrity, neuronal communication, and cytoskeletal scaffolding.

UniProt: O60941