TGM4 antibody (AA 1-684)

Catalog No. ABIN7869525

The Rabbit Polyclonal anti-TGM4 antibody is suitable to detect TGM4 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB and FACS.

Quick Overview for TGM4 antibody (AA 1-684) (ABIN7869525)

Target: TGM4 (Transglutaminase 4 (Prostate) (TGM4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGM4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-TGM4 Antibody

Binding Specificity: AA 1-684

Purpose: TGM4 Antibody / Transglutaminase 4

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TGM4 recombinant protein (Position: M1-K684) was used as the immunogen for the TGM4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TGM4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TGM4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TGM4

Target: TGM4 (Transglutaminase 4 (Prostate) (TGM4))

Alternative Name: TGM4

Background: TGM4 antibody detects Transglutaminase 4, an enzyme that catalyzes the cross-linking of proteins through ?-(gamma-glutamyl) lysine bonds, contributing to the stabilization of extracellular structures. The UniProt recommended name is Transglutaminase 4 (TGM4). This enzyme is part of the transglutaminase family, which mediates post-translational protein modification and plays critical roles in cell adhesion, differentiation, and reproductive physiology.

Functionally, TGM4 antibody identifies a 684-amino-acid secreted and membrane-associated protein primarily expressed in the prostate and other reproductive tissues. TGM4 cross-links seminal plasma proteins, forming insoluble networks that contribute to semen coagulation and fertility. It may also participate in epithelial barrier formation and immune defense in the urogenital tract.

The TGM4 gene is located on chromosome 3p21.33 and is expressed in male reproductive tissues and epithelial surfaces. TGM4 activity is regulated by calcium ions and thiol-reactive compounds, influencing enzyme conformation and catalytic activity.

Pathologically, altered TGM4 expression has been associated with prostate cancer and infertility. Decreased expression correlates with impaired seminal coagulation, while increased levels are observed in prostate tumors and serve as potential diagnostic biomarkers. Research using TGM4 antibody supports studies in enzymology, reproductive biology, and oncology.

TGM4 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect transglutaminase enzymes. NSJ Bioreagents provides TGM4 antibody reagents optimized for reproductive biology, enzymatic modification, and cancer biomarker studies.

Structurally, Transglutaminase 4 consists of a catalytic core domain containing the active site cysteine, histidine, and aspartate residues, flanked by beta-sandwich and C-terminal domains that mediate substrate binding. This antibody enables analysis of TGM4's role in cross-linking reactions and reproductive function.

UniProt: P49221