Axin antibody (AA 1-811)

Catalog No. ABIN7869571

The Rabbit Polyclonal anti-Axin antibody (ABIN7869571) specifically detects Axin in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for Axin antibody (AA 1-811) (ABIN7869571)

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Axin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Axin Antibody

Binding Specificity: AA 1-811

Purpose: AXIN1 Antibody / Axin-1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human AXIN1 recombinant protein (Position: M1-K811) was used as the immunogen for the AXIN1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the AXIN1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the AXIN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Axin

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: AXIN1

Background: AXIN1 antibody detects Axin-1, encoded by the AXIN1 gene on chromosome 16p13.3. AXIN1 antibody is widely applied in studies of Wnt signaling, cell fate determination, and cancer. Axin-1 is a scaffold protein that functions as a negative regulator of the canonical Wnt signaling pathway by promoting degradation of beta-catenin. It is essential for development, stem cell maintenance, and oncogenesis regulation. Through its multiple interaction domains, Axin-1 coordinates formation of the beta-catenin destruction complex, ensuring tight regulation of Wnt target gene expression.

Structurally, Axin-1 is a ~96 kDa cytoplasmic protein composed of an RGS domain, coiled-coil regions, and interaction motifs for APC, GSK3, and beta-catenin. These domains enable assembly of the multiprotein destruction complex. Axin-1 also binds Dishevelled, tankyrase, and other regulators that modulate Wnt activity. Alternative isoforms exist with altered binding properties, influencing Wnt signaling output.

Functionally, Axin-1 acts as a central scaffold in beta-catenin regulation. In the absence of Wnt ligands, Axin-1 recruits beta-catenin, APC, CK1, and GSK3 to promote phosphorylation and degradation of beta-catenin, suppressing transcriptional activation. Upon Wnt stimulation, Axin-1 is destabilized by tankyrase-mediated poly(ADP-ribosyl)ation, allowing beta-catenin accumulation and target gene activation. Researchers use AXIN1 antibody to study Wnt pathway regulation, developmental processes, and tumorigenesis.

Clinically, AXIN1 mutations and deletions have been identified in hepatocellular carcinoma, medulloblastoma, and colorectal cancer, underscoring its tumor suppressor role. Dysregulation of Axin-1 contributes to abnormal Wnt signaling, a hallmark of many cancers. AXIN1 is also implicated in developmental defects and stem cell differentiation. Because Wnt signaling is a therapeutic target, Axin-1 is under investigation as a biomarker and potential drug target. NSJ Bioreagents supplies AXIN1 antibody for Wnt signaling, cancer, and developmental research.

Experimentally, AXIN1 antibody is applied in western blotting to detect the ~96 kDa protein, in immunofluorescence microscopy to assess cytoplasmic puncta, and in immunohistochemistry to study tumor tissue. Co-immunoprecipitation with AXIN1 antibody identifies destruction complex partners and signaling regulators.

UniProt: O15169

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling