POLR2A/RPB1 antibody (AA 10-321)

Catalog No. ABIN7869698

This Rabbit Polyclonal antibody specifically detects POLR2A/RPB1 in WB, IF, ELISA, FACS and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for POLR2A/RPB1 antibody (AA 10-321) (ABIN7869698)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-POLR2A/RPB1 Antibody

Binding Specificity: AA 10-321

Purpose: POLR2A Antibody / RNA polymerase II subunit RPB1

Purification: Affinity purified

Immunogen: Recombinant human protein (amino acids D10-E321) was used as the immunogen for the POLR2A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the POLR2A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the POLR2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A

Background: DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. It is mapped to 17p13.1. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

UniProt: P24928

Pathways: Regulatory RNA Pathways