PRAME antibody (AA 100-250)

Catalog No. ABIN7869793

The Mouse Monoclonal anti-PRAME antibody (Clone PRAME-9188) (ABIN7869793) specifically detects PRAME in IHC (p). The antibody is reactive with Human samples.

Quick Overview for PRAME antibody (AA 100-250) (ABIN7869793)

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRAME antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PRAME-9188

Product Details anti-PRAME Antibody

Binding Specificity: AA 100-250

Purpose: PRAME Antibody / OIP-4

Purification: Protein A/G affinity

Immunogen: A recombinant fragment (within amino acids 100-250) of human PRAME protein was used as the immunogen for the PRAME antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the PRAME antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PRAME antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for PRAME

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Alternative Name: PRAME

Background: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.

UniProt: P78395

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding