TRIOBP antibody (AA 1079-2365)

Catalog No. ABIN7870018

The Rabbit Polyclonal anti-TRIOBP antibody (ABIN7870018) specifically detects TRIOBP in WB, IHC, ELISA, IF, FACS and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TRIOBP antibody (AA 1079-2365) (ABIN7870018)

Target: TRIOBP (TRIO and F-Actin Binding Protein (TRIOBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIOBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-TRIOBP Antibody

Binding Specificity: AA 1079-2365

Purpose: TRIOBP Antibody / Trio and F-actin-binding protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TRIOBP recombinant protein (Position: H1079-E2365) was used as the immunogen for the TRIOBP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TRIOBP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TRIOBP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TRIOBP

Target: TRIOBP (TRIO and F-Actin Binding Protein (TRIOBP))

Alternative Name: TRIOBP

Background: TRIOBP antibody detects Trio and F-actin-binding protein, encoded by the TRIOBP gene on chromosome 22q13.1. TRIOBP antibody is widely used in studies of cytoskeletal dynamics, hearing, and neurodevelopment. TRIOBP is an actin-binding protein involved in organizing filamentous actin into stable bundles, regulating cell shape, adhesion, and structural integrity. Isoforms of TRIOBP have tissue-specific functions, particularly in the inner ear where they are essential for stereocilia architecture in sensory hair cells.

Structurally, TRIOBP is a large protein of ~236 kDa in its longest isoform, but smaller isoforms such as TRIOBP-1 and TRIOBP-4 exist due to alternative splicing. It contains coiled-coil regions and actin-binding motifs, which facilitate F-actin crosslinking. The presence of multiple isoforms allows functional diversity, with long forms stabilizing actin filaments and shorter ones regulating cytoskeletal assembly dynamics.

Functionally, TRIOBP stabilizes actin bundles in stereocilia of cochlear hair cells, supporting mechanotransduction required for hearing. Knockdown or mutation of TRIOBP results in stereocilia disorganization and deafness. In neurons, TRIOBP contributes to dendritic spine morphology and synaptic plasticity. It is also implicated in stress fiber formation and cancer cell invasion. Researchers use TRIOBP antibody to study actin regulation, auditory biology, and neuronal development.

Clinically, TRIOBP mutations cause autosomal recessive nonsyndromic deafness (DFNB28), reflecting its essential role in stereocilia maintenance. Variants are also associated with psychiatric disorders including schizophrenia, likely due to its influence on synaptic architecture. Dysregulated TRIOBP expression has been observed in cancers, particularly glioblastoma, where it promotes invasion and survival. NSJ Bioreagents provides TRIOBP antibody for cytoskeletal biology, auditory research, and cancer studies.

Experimentally, TRIOBP antibody is used in western blotting to detect isoforms of varying sizes, in immunofluorescence to visualize actin bundle association, and in immunohistochemistry to analyze cochlear and neural tissue. Co-immunoprecipitation with TRIOBP antibody identifies actin and cytoskeletal regulators as interaction partners.

UniProt: Q9H2D6

Pathways: Regulation of Actin Filament Polymerization