LIMS1 antibody (AA 11-172)

Catalog No. ABIN7870068

The Rabbit Polyclonal anti-LIMS1 antibody is suitable to detect LIMS1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for LIMS1 antibody (AA 11-172) (ABIN7870068)

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIMS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-LIMS1 Antibody

Binding Specificity: AA 11-172

Purpose: LIMS1 Antibody / LIM and senescent cell antigen-like-containing domain protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human LIMS1 recombinant protein (Position: E11-A172) was used as the immunogen for the LIMS1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the LIMS1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the LIMS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for LIMS1

Target: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Alternative Name: LIMS1

Background: LIMS1 antibody detects LIM and senescent cell antigen-like-containing domain protein 1, encoded by the LIMS1 gene on chromosome 2q12.3. LIMS1 antibody is widely applied in studies of integrin signaling, cell adhesion, and cancer biology. LIMS1, also known as PINCH1, is a LIM domain-containing adaptor protein that localizes to focal adhesions, where it connects integrins with the actin cytoskeleton and signaling pathways. It plays a central role in cell-matrix interactions and regulation of survival, proliferation, and migration.

Structurally, LIMS1 is a ~37 kDa protein composed of five LIM domains, which mediate protein-protein interactions. Through these domains, LIMS1 interacts with integrin-linked kinase (ILK) and parvin to form the IPP complex, a key signaling hub at focal adhesions. This complex links extracellular matrix adhesion with intracellular signaling cascades.

Functionally, LIMS1 stabilizes focal adhesions and promotes integrin-mediated signaling. It regulates pathways including PI3K-Akt, MAPK, and Rho GTPase signaling, influencing survival, motility, and cytoskeletal organization. Knockout models demonstrate that LIMS1 is essential for embryonic development and cell viability. Researchers use LIMS1 antibody to investigate adhesion signaling, integrin function, and tumor progression.

Clinically, LIMS1 is associated with cancer, where its overexpression promotes invasion and metastasis by enhancing integrin signaling. It also influences therapy resistance in multiple tumor types. Genetic studies have linked LIMS1 variants to immune function and kidney transplantation outcomes, suggesting roles beyond adhesion. NSJ Bioreagents supplies LIMS1 antibody for use in adhesion biology, immunology, and oncology.

Experimentally, LIMS1 antibody is applied in western blotting to detect the ~37 kDa protein, in immunofluorescence to visualize focal adhesion localization, and in immunohistochemistry to assess tumor expression. Co-immunoprecipitation with LIMS1 antibody identifies ILK, parvin, and integrin-binding partners.

UniProt: P48059