REST antibody (AA 110-1097)

Catalog No. ABIN7870097

This Rabbit Polyclonal antibody specifically detects REST in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for REST antibody (AA 110-1097) (ABIN7870097)

Target: REST (RE1-Silencing Transcription Factor (REST))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-REST Antibody

Binding Specificity: AA 110-1097

Purpose: REST Antibody / RE1-silencing transcription factor

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human REST recombinant protein (Position: E110-E1097) was used as the immunogen for the REST antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the REST antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the REST antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: REST

Background: REST antibody detects RE1-silencing transcription factor, a zinc finger DNA-binding protein that represses neuronal gene expression in non-neuronal cells. The UniProt recommended name is RE1-silencing transcription factor (REST). Also known as neuron-restrictive silencer factor (NRSF), this protein plays a pivotal role in epigenetic regulation of gene expression by binding to RE1/NRSE motifs and recruiting chromatin-modifying complexes.

Functionally, REST antibody identifies a 1097-amino-acid nuclear protein that suppresses transcription by forming repressive chromatin structures. REST recognizes RE1 sites present in the promoters of neuronal genes and recruits corepressors such as CoREST, mSin3, and histone deacetylases (HDAC1/2). These interactions promote chromatin condensation and transcriptional silencing, preventing inappropriate neuronal gene expression in non-neuronal tissues.

The REST gene is located on chromosome 4q12 and encodes a multi-zinc finger transcription factor with eight C2H2 zinc finger motifs that mediate sequence-specific DNA binding. REST acts as a master regulator of neurogenesis, ensuring proper timing of neuronal differentiation. During development, REST levels decline in differentiating neurons, allowing the expression of genes required for synaptic function, ion channel activity, and neurotransmitter release.

In addition to developmental regulation, REST participates in neural plasticity, stem cell maintenance, and stress response pathways. Dysregulation of REST has been linked to neurological disorders, including epilepsy, Huntington's disease, and Alzheimer's disease. Elevated REST expression in non-neuronal cancers such as medulloblastoma and neuroblastoma suggests a role in tumorigenesis through repression of differentiation pathways.

REST antibody is widely used in neuroscience, epigenetics, and cancer research. It is suitable for chromatin immunoprecipitation (ChIP), immunoblotting, and immunofluorescence to study REST localization, DNA binding, and interaction networks. This antibody aids in exploring transcriptional silencing mechanisms and neurodevelopmental regulation. In oncology, REST serves as a biomarker of altered differentiation and epigenetic reprogramming.

Structurally, REST contains an N-terminal repression domain that recruits mSin3-HDAC complexes and a C-terminal domain that binds CoREST and associated enzymes such as LSD1. REST-mediated repression involves coordinated histone deacetylation and demethylation, establishing a transcriptionally repressive chromatin environment. NSJ Bioreagents provides REST antibody reagents validated for use in transcriptional repression, neuronal gene regulation, and chromatin remodeling studies.

UniProt: Q13127

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity